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重組激活基因2蛋白抗體(ti)
| 產品名稱 | 英文名稱 | 貨號 |
| 重組激活基因2蛋白抗體 | RAG2 | GOY-01K0001 |
商品屬性：
英文名稱:RAG2
中文名:重組激活基因2蛋白抗體(ti)
別 名:RAG 2; RAG-2; RAG2; RAG2_HUMAN; Recombination activating gene 2; V(D)J recombination activating protein 2; V(D)J recombination-activating protein 2.
研究領域細胞生物: 免疫學 表觀遺傳(chuan) 學
抗體(ti) 來源:Rabbit
克隆類型:Polyclonal
交叉反應:(predicted: Human, Mouse, Rat, Dog, Pig, Cow, Horse, Rabbit, Sheep, )
產(chan) 品應用:ELISA=1:5000-10000 IHC-P=1:100-500 IHC-F=1:100-500 IF=1:100-500 （石蠟切片需做抗原修複）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理論分子量:58kDa
細胞定位細胞核
性 狀:Liquid
濃 度:1mg/ml
免 疫 原:KLH conjugated synthetic peptide derived from human RAG2: 451-527/527
亞(ya) 型:IgG
純化方法:affinity purified by Protein A
緩 衝(chong) 液:0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存條件:Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事項:This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMedPubMed
產(chan) 品介紹:
| Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. The chromatin structure plays an essential role in the V(D)J recombination reactions and the presence of histone H3 trimethylated at 'Lys-4' (H3K4me3) stimulates both the nicking and haipinning steps. The RAG complex also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B lymphocytes. The introduction of DNA breaks by the RAG complex on one immunoglobulin allele induces ATM-dependent repositioning of the other allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. In addition to its endonuclease activity, RAG1 also acts as a E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination. Mediates polyubiquitination of KPNA1. |
多克隆抗體(ti) 製備：
我公司合成抗原，保證陽性血清和抗體(ti) ELISA效價(jia) 達到1：32000，由蛋白抗原製備的多抗，保證WB檢測結果是陽性。技術服務實行分步報價(jia) ，按節點收費，為(wei) 客戶節約成本。
鼠單克隆抗體(ti) 製備:
客戶可根據項目實際需求情況靈活選擇進行，我公司將為(wei) 您的項目提供專(zhuan) 業(ye) 的建議。在製備過程中，與(yu) 客戶互動驗證，確保最終製備的抗體(ti) ELISA陽性血清檢測達到合同要求的同時，也能滿足客戶抗體(ti) 最終應用驗證需求。技術服務實行分步報價(jia) ，按節點收費，為(wei) 客戶節約成本。
重組抗體(ti) 表達服務:
重組抗體(ti) 技術使製備人源化抗體(ti) 和人源抗體(ti) 成為(wei) 可能，而這是其他常規的多克隆或單克隆抗體(ti) 製備方法無法做到的。我公司已搭建好的抗體(ti) 測序、重組抗體(ti) 表達等先進平台，能為(wei) 客戶提供各種類型的重組抗體(ti) 表達服務，包含鼠源單克隆抗體(ti) 構建及表達、更換恒定區亞(ya) 型的嵌合抗體(ti) 表達，抗體(ti) 人源化改造及表達等服務。
單項免疫學及檢測服務:
我公司也為(wei) 客戶提供單項免疫學和檢測服務，如動物免疫、動物飼養(yang) 、抗體(ti) 修飾服務（如生物素，FITC，HRP等的標記）、以及ELISA，WB，IHC，DB等檢測服務。
抗體(ti) 定製服務:
已注冊(ce) 商標，主要用於(yu) 抗體(ti) 藥物發現和開發，是我公司針對有特定應用要求和各種模型測試的高端客戶量身定製的專(zhuan) 業(ye) 服務，主要麵向工業(ye) 客戶。
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